ISOLATION AND CHARACTERISATION OF LYMPHOCYTES Lab Report

ISOLATION AND CHARACTERISATION OF LYMPHOCYTES - Lab Report manakinThis is because this results in an inaccurate count. The same is true if the cover slip is moved after the sample is loaded. To deposit randomized counting only the 4 squargons at each(prenominal) end were picked and then the 16 thin squares within each large square, that is, in an organized zigzag a huge the rows. To make the counting like only booths lying on the bottom and right hand boundary were counted in and a cell lying on the top or left hand boundary were not counted in. This was in revisal to avoid counting cells twice. Normally, at least two squares should be counted, comprising to a greater extent than 100 cells within each central counting area of every square. For higher accuracy, additional cells can be counted and the mean employ to compute cell concentration. The result of cells counts were in the table 1 below.The other cells in the splenocyte preparation are red cells 3.82%, Granulocytes 6.1 % as shown in the table 4 above. The abundance of small lymphocytes shows that the cell suspension has more immature cells which are maturing in the spleen to become large lymphocytes and T-lymphocytes. With time the population of small lymphocytes decreases as they develop to large lymphocytes, medium lymphocytes and T-lymphocytes. The spleen is made up of immune lymphocytes and that why in this examine there were low number of other types of cells. Time was very important in this science lab because long exposure could lead to lack of the reliability. This is because the population of splenocytes keep on changing since some cell has not matured fully and other are multiplying. For this lab the time limit was hour sinceIn this lab exercise, Nigrosin was used to stain any dead cells. Nigrosin is an acidic stain. This means the stain readily gives up a hydrogen ion and becomes negatively charged. Since the surface of dead cells is negatively charged, the cell surface repels the sta in. Thus slide sugarcoat will stain, but the dead cell will not. The dead